BIOCHEMISTRY THESIS TOPICS FOR MD/DNB

1. Enzyme Kinetics and Regulation

1. A cross-sectional observational study of the inhibitory effects of heavy metal ions on alkaline phosphatase activity in human serum samples

2. A comparative evaluation of substrate specificity constants of recombinant acetylcholinesterase enzymes using Michaelis-Menten kinetic analysis in vitro

3. An observational time-course analysis of lactate dehydrogenase reaction rates in type 2 diabetes mellitus patients

4. A cross-sectional study of pH and temperature dependence of α-amylase activity in salivary samples of healthy volunteers

5. A comparative assessment of competitive versus non-competitive inhibition kinetics of 3-hydroxy-3-methylglutaryl coenzyme A reductase by sulfonamide derivatives

6. A cross-sectional observational analysis of creatine kinase release kinetics in athletes following acute exercise-induced muscle injury

7. A comparative kinetic study of glucose-6-phosphate dehydrogenase variants in patients with glucose-6-phosphate dehydrogenase deficiency

8. A cross-sectional investigation of urease inhibition kinetics by plant-derived phytochemicals in vitro

9. A comparative analysis of Michaelis-Menten constants of human pancreatic lipase isolated from healthy versus hyperlipidemic subjects

10. A cross-sectional observational study of the effect of statin therapy on 3-hydroxy-3-methylglutaryl coenzyme A reductase activity in dyslipidemic patients

2. Metabolic Pathway Alterations in Disease

1. A cross-sectional observational study of glycolytic enzyme activities in skeletal muscle biopsies of type 2 diabetes mellitus patients

2. A comparative analysis of serum tricarboxylic acid cycle metabolite concentrations in acute myocardial infarction patients versus healthy controls

3. A cross-sectional observational study of urea cycle enzyme deficiencies in children presenting with hyperammonemia

4. A comparative evaluation of pentose phosphate pathway flux in erythrocytes of sickle cell disease patients and healthy volunteers

5. A cross-sectional study of fatty acid β-oxidation enzyme activities in liver biopsy specimens of nonalcoholic fatty liver disease patients

6. A comparative study of polyol pathway enzyme aldose reductase activity in diabetic versus non-diabetic subjects

7. A cross-sectional observational assessment of one-carbon metabolism markers in pregnant women with neural tube defect pregnancies

8. A comparative evaluation of purine degradation metabolite profiles in gout patients and asymptomatic hyperuricemic individuals

9. A cross-sectional study of phosphatidylinositol pathway intermediate levels in metabolic syndrome patients

10. A comparative observational study of bile acid synthesis pathway markers in cholestatic liver disease versus normal liver function subjects

3. Molecular Techniques in Biochemistry

1. A cross-sectional observational study of pro-inflammatory cytokine gene expression in rheumatoid arthritis synovial fluid using quantitative real-time polymerase chain reaction

2. A comparative evaluation of apoptotic marker protein expression in breast cancer tissue samples using Western blot analysis

3. A cross-sectional study of serum antioxidant enzyme levels in chronic kidney disease patients by enzyme-linked immunosorbent assay

4. A comparative assessment of microRNA signatures in peripheral blood mononuclear cells of diabetic nephropathy patients using next-generation sequencing

5. A cross-sectional observational analysis of mitochondrial DNA mutations in mitochondrial myopathy patients by polymerase chain reaction and Sanger sequencing

6. A comparative study of gene editing efficiency of clustered regularly interspaced short palindromic repeats-associated protein 9 mediated knockout of the p53 gene in human cell lines

7. A cross-sectional study of transcription factor binding dynamics in leukemic cells using chromatin immunoprecipitation assay

8. A comparative evaluation of virus–host protein interactions using co-immunoprecipitation techniques

9. A cross-sectional assessment of tumor suppressor gene promoter methylation status in colorectal cancer tissues by methylation-specific polymerase chain reaction

10. A comparative observational study of multiplex bead-based immunoassay cytokine profiling in cerebrospinal fluid of multiple sclerosis patients versus healthy volunteers

4. Biomarkers in Clinical Biochemistry

1. A cross-sectional observational study of serum cardiac troponin I levels in acute coronary syndrome patients compared to stable angina controls

2. A comparative evaluation of serum procalcitonin levels in bacterial sepsis versus viral sepsis patients

3. A cross-sectional study of plasma C-reactive protein and interleukin-6 levels in rheumatoid arthritis patients

4. A comparative analysis of serum cancer antigen 125 levels in ovarian cancer patients versus benign ovarian cyst cases

5. A cross-sectional assessment of urinary albumin-to-creatinine ratio in various stages of diabetic nephropathy

6. A comparative observational study of plasma D-dimer fibrin degradation product levels in deep vein thrombosis patients versus healthy individuals

7. A cross-sectional study of cerebrospinal fluid beta-amyloid and tau protein levels in Alzheimer’s disease patients compared to cognitively normal controls

8. A comparative analysis of serum vitamin D and parathyroid hormone levels in osteoporosis patients and age-matched healthy volunteers

9. A cross-sectional observational evaluation of serum ferritin and transferrin saturation in iron deficiency anemia versus anemia of chronic disease

10. A comparative study of serum lactate levels in septic shock versus non-septic critically ill patients

5. Nutritional and Lipid Biochemistry

1. A cross-sectional observational study of serum lipid profile differences between vegetarians and non-vegetarians in a coastal population

2. A comparative evaluation of serum vitamin D and calcium levels in postmenopausal women with and without osteoporosis

3. A cross-sectional analysis of micronutrient deficiencies in malnourished children attending rural health clinics

4. A comparative observational study of the effects of omega-3 fatty acid supplementation on serum triglyceride levels in hypertriglyceridemia patients

5. A cross-sectional study of dietary antioxidant intake and plasma oxidative stress markers in metabolic syndrome patients

6. A comparative analysis of enteral versus parenteral nutrition impact on serum protein levels in critically ill patients

7. A cross-sectional observational evaluation of serum vitamin B12 levels and cognitive function correlation in elderly subjects

8. A comparative study of lipid peroxidation and antioxidant enzyme activities in smokers versus non-smokers

9. A cross-sectional study of high-protein diet effects on renal function biomarkers in athletes

10. A comparative observational analysis of serum phospholipid fatty acid composition in coronary artery disease patients and healthy controls

6. Genetic and Molecular Basis of Diseases

1. A cross-sectional study of apolipoprotein E gene polymorphisms in Alzheimer’s disease patients and age-matched controls

2. A comparative observational analysis of breast cancer type 1 susceptibility protein gene and breast cancer type 2 susceptibility protein gene mutation prevalence in Indian breast cancer patients

3. A cross-sectional study of angiotensin-converting enzyme gene single nucleotide polymorphisms among hypertensive patients

4. A comparative analysis of human leukocyte antigen B27 antigen frequency in ankylosing spondylitis patients versus healthy individuals

5. A cross-sectional observational assessment of cystic fibrosis transmembrane conductance regulator gene mutations in suspected cystic fibrosis cases

6. A comparative study of Kirsten rat sarcoma viral oncogene homolog gene mutation status in colorectal cancer tissue versus adjacent normal mucosa

7. A cross-sectional study of vitamin D receptor gene polymorphisms in tuberculosis patients

8. A comparative observational evaluation of mitochondrial DNA haplogroups in Leber’s hereditary optic neuropathy patients versus healthy relatives

9. A cross-sectional study of tumor necrosis factor alpha promoter polymorphisms in psoriasis patients

10. A comparative study of insulin receptor substrate gene expression levels in type 2 diabetes mellitus patients

7. Environmental and Toxicological Biochemistry

1. A cross-sectional observational study of serum lead and cadmium levels in battery manufacturing workers versus office employees

2. A comparative analysis of urinary pesticide metabolite biomarkers in agricultural workers and non-agricultural controls

3. A cross-sectional study of blood mercury levels in fish-consuming coastal populations

4. A comparative observational evaluation of acetylcholinesterase activity in agricultural workers exposed to organophosphate pesticides versus unexposed individuals

5. A cross-sectional study of serum lipid peroxidation markers in residents living near industrial effluent discharge sites

6. A comparative analysis of liver enzyme levels in petrochemical workers exposed to benzene versus administrative staff

7. A cross-sectional observational study of urinary arsenic species in villagers using contaminated groundwater

8. A comparative study of antioxidant enzyme activities in children from urban versus rural environments with differing air pollution levels

9. A cross-sectional analysis of DNA adduct formation in blood lymphocytes of smokers compared to non-smokers

10. A comparative observational analysis of serum organochlorine pesticide residues in mothers and their newborn infants

8. Signal Transduction and Cell Communication

1. A cross-sectional observational study of phosphorylation levels of mitogen-activated protein kinase in synovial fluid of rheumatoid arthritis patients

2. A comparative analysis of insulin receptor substrate phosphorylation in adipose tissue of obese versus lean individuals

3. A cross-sectional study of intracellular cyclic adenosine monophosphate levels in lymphocytes of asthma patients compared to healthy controls

4. A comparative observational evaluation of nuclear factor kappa-light-chain-enhancer of activated B cells activation in mononuclear cells of sepsis patients versus healthy volunteers

5. A cross-sectional study of calcium signaling dynamics in platelets of patients with bleeding disorders

6. A comparative analysis of receptor tyrosine kinase expression in malignant versus benign thyroid nodules

7. A cross-sectional observational assessment of Notch signaling pathway component levels in keratinocytes of psoriatic lesions

8. A comparative study of G protein-coupled receptor desensitization rates in heart failure patients versus healthy subjects

9. A cross-sectional study of phosphoinositide 3-kinase activity in muscle biopsy specimens from muscular dystrophy patients

10. A comparative observational evaluation of Janus kinase and signal transducer and activator of transcription pathway activation in blood cells of leukemia patients and healthy donors

9. Proteomics and Metabolomics

1. A cross-sectional observational study of serum proteomic profiles in early-stage ovarian cancer patients using mass spectrometry

2. A comparative analysis of urinary metabolomic fingerprints in diabetic nephropathy patients and healthy controls

3. A cross-sectional study of cerebrospinal fluid proteomic alterations in Alzheimer’s disease using two-dimensional gel electrophoresis

4. A comparative observational evaluation of plasma metabolomic signatures in acute myocardial infarction patients versus stable angina subjects

5. A cross-sectional study of salivary proteome changes in periodontal disease patients compared to healthy volunteers

6. A comparative analysis of tissue metabolomic profiles in invasive versus in situ breast carcinoma

7. A cross-sectional observational assessment of serum lipidomic changes in non-alcoholic steatohepatitis patients using liquid chromatography-mass spectrometry

8. A comparative study of exhaled breath metabolomic patterns in asthmatic versus non-asthmatic individuals

9. A cross-sectional analysis of urine proteomics in chronic kidney disease patients using matrix-assisted laser desorption ionization time-of-flight mass spectrometry

10. A comparative observational study of fecal metabolomic profiles in inflammatory bowel disease patients versus healthy controls

10. Recent Studies in Biochemistry

1. A cross-sectional observational study of gut microbiota-derived short-chain fatty acid levels in ulcerative colitis patients

2. A comparative evaluation of clustered regularly interspaced short palindromic repeats-associated protein 9 base editing efficiency for correcting β-globin gene mutations in patient-derived induced pluripotent stem cells

3. A cross-sectional observational analysis of SARS-CoV-2 spike protein–antibody binding kinetics in vaccinated versus naturally infected individuals

4. A comparative study of nanoparticle-mediated targeted drug delivery efficacy in cancer cell cultures versus conventional chemotherapy

5. A cross-sectional assessment of cerebrospinal fluid metabolomic alterations in neurodegenerative disorder patients using high-resolution mass spectrometry

6. A comparative observational study of machine learning–based protein folding prediction versus conventional homology modeling approaches

7. A cross-sectional study of post-translational modification patterns in histone proteins of acute leukemia patients using mass spectrometry

8. A comparative evaluation of messenger RNA vaccine stability at varying storage temperatures using biochemical and biophysical assays

9. A cross-sectional observational analysis of extracellular vesicle proteome changes in septic patients compared to healthy individuals

10. A comparative study of synthetic biology approaches for enzymatic pathway engineering to enhance biofuel production in microbial systems